In order to reflect the expected developmental potential of the female and male gametes (EGP), the profiles of the couples undergoing fertility treatment were classified into three categories: “below EGP, “EGP” and “above EGP”. To this end, the following parameters are used, among others, for prognosis:
- Age of the couples
- Specific stimulation protocol
- Precise sperm analysis (IMSI - Intracytoplasmic morphological selected sperm injection)
- Fertilization rate
- Specific protocol for blastocyst culture
- High quality standard of the laboratories in the IVF Centers Prof. Zech
- Vitrification technique
Case A: 33-year-old female with a 42-year-old male partner, mild oligozoospermia, and secondary idiopathic sterility after biochemical pregnancy.
Cycle outcome:7 oocytes obtained at pick-up; 6 mature oocytes; all 6 were fertilized (FR 100%); 5 good quality blastocysts on day 5; transfer of 2 blastocysts (Bl 5BA, Bl 4AB) and birth of one boy; and cryopreservation of 3 blastocysts.
Semen analysis: 2.6 ml; 11 Mio/ml; progressive motility: 57%; and IMSI report: 8% Class I, 51% Class II, and 41% Class III sperm.
Case B: 35-year-old female with a 38-year-old male partner, normozoospermia, and primary sterility by obstruction of the fallopian tubes.
Cycle outcome:11 oocytes obtained at pick-up; 10 mature oocytes; all fertilized (FR 100%); 1 blastocyst (fBl c); transfer of 1 blastocyst; no pregnancy in this cycle.
Semen analysis:3.5 ml; 18.4 Mio/ml; progressive motility: 55%; and IMSI report: 0% Class I, 25% Class II, and 75% Class III sperm.
Case C: 34-year-old female with a 37-year-old male partner, paraplegic, oligoasthenozoospermia, and primary sterility.
Cycle outcome:16 oocytes obtained at pick-up; 13 mature oocytes; 12 fertilized (FR 92.3%); 5 blastocysts (4 good quality and 1 very low quality); transfer of 1 blastocyst (Bl 2AB) and birth of one healthy girl; and cryopreservation of 3 blastocysts.
Semen analysis:1 ml; 2.5 Mio/ml; progressive motility: 5%; and IMSI report: 0% Class I, 11% Class II, and 89% Class III sperm.
[Figure 1: Classification of cases A, B, and C into prognosis groups based on number of oocytes (poor responder: 1-8 oocytes, normal responder: 9-12 oocytes, or high responder: >12 oocytes), fertilization rate FR (%) classified as normal between 80-100%, or blastocyst outcome on day 5 (below EGP: 1-3 blastocysts, EGP: 4-5 blastocysts, or above EGP: >5 blastocysts).]